rabbit anti gal3  (Novus Biologicals)

Journal: Food Science & Nutrition

Article Title: Pinellia pedatisecta Schott‐Derived Exosome‐Like Nanovesicles Promote Apoptosis in Colorectal Cancer by Regulating the Lysosome‐Mediated Mitophagy Pathway

doi: 10.1002/fsn3.71500

Figure Lengend Snippet: Lysosome inhibitor suppressed mitophagy in PPS‐ELNs‐treated HCT116 cells. (A) The levels of ROS, SOD, and MDA were detected by commercial kits. (B) Autophagosomes in HCT116 cells were observed by TEM. Scale bar = 1 μm. (C) Lysosomes in HCT116 cells were detected by LysoTracker Red staining. Scale bar = 100 μm. (D) The co‐localization of LC3 and mitochondrial protein TOM20 was detected by immunofluorescence. Scale bar = 100 μm. (E) The expression of p62, PINK1, Parkin, Gal3, TOM20, and LC3‐II/I was detected by Western blot. ** p < 0.01 vs. Control group. # p < 0.05, ## p < 0.01 vs. PPS‐ELNs group. CQ, chloroquine.

Article Snippet: Subsequently, sections were incubated with primary antibodies including Ki67 (1:300, Affinity, AF0198) and Gal3 (1:100, CST, 87985) at 4°C overnight.

Techniques: Staining, Immunofluorescence, Expressing, Western Blot, Control

Journal: Food Science & Nutrition

Article Title: Pinellia pedatisecta Schott‐Derived Exosome‐Like Nanovesicles Promote Apoptosis in Colorectal Cancer by Regulating the Lysosome‐Mediated Mitophagy Pathway

doi: 10.1002/fsn3.71500

Figure Lengend Snippet: PPS‐ELNs regulated lysosome‐mediated mitophagy pathway in CRC mice. (A) The expression of Gal3 in tumors was detected by immunohistochemistry. Scale bar = 20 μm. (B) Autophagosomes in tumor tissues were observed by TEM. Scale bar = 1 μm. (C) The expression of p62, TOM20, LC3‐II/I, PINK1, and Parkin was detected by Western blot. * p < 0.05, ** p < 0.01 vs. Control group.

Article Snippet: Subsequently, sections were incubated with primary antibodies including Ki67 (1:300, Affinity, AF0198) and Gal3 (1:100, CST, 87985) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry, Western Blot, Control