rabbit anti gal3  (Novus Biologicals)

Journal: EMBO Reports

Article Title: An ATG12‐ATG5‐TECPR1 E3‐like complex regulates unconventional LC3 lipidation at damaged lysosomes

doi: 10.15252/embr.202356841

Figure Lengend Snippet: A, B Representative live‐cell fluorescent images of HeLa cells co‐transfected with EGFP‐TECPR1 and mCherry‐Gal3 and treated with 1 mM LLOMe for the indicated time. Arrowheads in (B) indicate the appearance of TECPR1 (green arrow) and Gal3 (magenta arrow). Scale bars = 10 μM for (A) and 1 μM for (B). C Quantification of TECPR1 and Gal3 recruitment to damaged lysosomes. Data are presented as mean ± SD from five independent experiments (each experiment represents a single cell with at least three individual lysosomes quantified). D Representative confocal image of a HeLa cell transfected with EGFP‐TECPR1, treated with LLOMe for 5 min, and immunostained for CHMP2A and LAMP1. Scale bars = 10 μm for whole image and 1 μm for insets. Fluorescence intensity profiles of the indicated channels across the dotted lines are shown in the lower subpanel. E Representative confocal images of a HeLa cells transfected with EGFP‐TECPR1 and TagBFP‐TMEM192 (a lysosomal/late endosomal protein), treated with 1 mM LLOMe for the indicated time, and immunostained for ALIX and Gal3. Scale bars = 10 μm. Source data are available online for this figure.

Article Snippet: Antibodies used in this study were from the following sources: LC3A (#4599, WB: 1:1,000), LC3B (#2775, WB: 1:1,000), GABARAP (#13733, WB: 1:1,000), GABARAPL1 (#26632, WB: 1:1,000), ATG16L1 (#8089, WB: 1:1,000), FIP200 (#12436, WB: 1:1,000), ATG7 (#8558, WB: 1:1,000), ATG12—mouse‐specific (#2011, WB: 1:1,000), TECPR1 (#8097, WB: 1:1,000), LAMP1 (#15665, IF: 1:100) and Gal3 (#87985, IF: 1:400) antibodies were purchased from Cell Signaling Technology.

Techniques: Transfection, Fluorescence

Journal: Dental materials journal

Article Title: Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.

doi: 10.4012/dmj.2016-295

Figure Lengend Snippet: Fig. 8 Immunohistologic staining of CD68 and gal3 (A, B, and C), P4HB (D, E, and F), ASMA (G, H, and I), RECA-1 (J, K, and L), and peroxidase activity (PA) (M, N, and O) in rat subcutaneous tissue at 10 days for implanted COL (A, D, G, J, and M), TCP5 (B, E, H, K, and N), and TCP25 (C, F, I, L, and O). CD68, P4HB, ASMA and RECA-1 are shown in red, and gal3 and the nucleus of all cells are shown in green. Arrows indicate peroxidase-positive neutrophils. Scale bars respectively represent 100 µm (A–L) and 20 μm (M–O).

Article Snippet: After pretreatment with 0.3% Triton X-100 and normal donkey serum, the sections were incubated overnight with the following primary antibodies, alone or in mixtures as indicated in the data: mouse antiCD68 (1:100 dilution; AbD Serotec, Kidlington, UK), rabbit anti-galectin3 (gal3) (1:300 dilution, sc-20157; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-prolyl-4-hydroxylase beta (P4HB) (1:1600 dilution, clone6-9H6; Acris Antibodies, San Diego, CA, USA), mouse anti-rat endothelial cell antigen-1 (RECA1) (1:1000 dilution; AbD Serotec), and mouse antialpha-smooth muscle actin (ASMA) (1:1600 dilution, clone 1A4; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Staining, Activity Assay